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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two added splice variants were described but specific nucleotide sequence details is not nevertheless accessible.

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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized from the antibody.

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This manual outlines further more achievable optimization of cellular and immunoassay parameters to be certain the absolute best outcomes are...

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This tutorial outlines further possible optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...

Antibodies bound to the blocking peptide not bind on the epitope about the focus on protein. check here By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is unique.

Focus on Information and facts Cyclin E belongs towards the extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation designs which lead into the temporal coordination of each and every mitotic event. Cyclin E kinds a fancy with and features for a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary which is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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